Bacterial RNA
From Health Encyclopedia
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Scope Note
ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
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Facts (generated by robot; please edit if you find it inaccurate)
- Frequently too little bacterial RNA is present for generating adequate signals on microrarrays.
- Bacterial RNA is not poly-adenylated (the RNA doesn't have poly A tails), so it cannot be primed for reverse transcription using the oligo dT primer in the Clontech PCR-Select Kit.
- Although substantial developments have recently occurred in the determination of numerous complete bacterial genome sequences, analysis of bacterial genome function by direct examination of bacterial RNA is inherently difficult due to the nature of prokaryotic mRNA.
- However, DNA microarrays require substantial amounts of RNA to generate the probes, especially when bacterial RNA is used for hybridization (50 microg of bacterial total RNA contains approximately 2 microg of mRNA).
- The conserved structure of a bacterial RNA is depicted as a backbone.
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