Dideoxynucleotide

Use

The strand synthesis reaction continues until a dideoxynucleotide is added, blocking further elongation.
Each dideoxynucleotide is labeled with a different fluorescent compound so that it will give off an identifying color in a laser beam.
However, if a dideoxynucleotide is joined to a base, then that fragment of DNA can no longer be elongated since a dideoxynucleotide lacks a crucial 3'-OH group.
The DNA fragment in the sequencing reaction becomes dye-labeled when a dideoxynucleotide is incorporated.
You are here: Home : Help : A Glossary of Terms : Dideoxynucleotide Dideoxynucleotide A modified nucleotide that is similar to the nucleotides used to make DNA, but that contains one less oxygen atom.
Mechanism-based Suppression of Dideoxynucleotide Resistance by K65R Human Immunodeficiency Virus Reverse Transcriptase Using an -Boranophosphate Nucleoside Analogue* Boulbaba Selmi, Jolle Boretto, Simon R.
But because a nucleotide-like compound called a dideoxynucleotide triphosphate – ddC, ddG, ddA or ddT – also is included, Taq stops copying when it incorporates that compound, like a train that halts on the track when it hits a rock.
tuberculosis enzyme were unable to discriminate against dideoxynucleotide 5'-triphosphates and were thus efficiently inhibited by these chain-terminating nucleotide analogues during DNA synthesis.

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