FRAP (fluorescence recovery after photobleaching)
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fluorescence recovery after photobleaching
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Facts (generated by robot; please edit if you find it inaccurate)
- FRAP (Fluorescence Recovery After Photobleaching) Conventional FRAP applications are performed using a laser controlled via the AOTF (Acousto-Optical Tuneable Filters).
- As determined by FRAP (fluorescence recovery after photobleaching), the lateral mobility of GFP–Dad1-tagged TCs was much more restricted than expected from the estimated size of the TC and can be affected by the functional state of the TCs.
- We recently investigated this possibility using FRAP (fluorescence recovery after photobleaching) to measure the diffusion coefficient of a membrane-bound GFP (green fluorescent protein) within the inner leaflet of the plasma membrane [28].
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