Gel retardation assay

Use

gel mobility shift assay

Facts (generated by robot; please edit if you find it inaccurate)

• The fluorescence gel-retardation assay is used to quantify the interaction between S pro- tein and S15.
• The fluorescence gel retardation assay is a rapid method to demonstrate the existence of a protein-protein interaction and to estimate the dissociation constant (Kd) of the resulting complex.
• To exclude the possibility that p53CP observed in the gel retardation assay is an alternate form of p53, we used antibody against alternatively spliced p53 (34) and found that the antibody neither blocked nor supershifted p53CP (data not shown).
• The same type of interaction that is used for gel retardation assay is used except you are looking for binding to a clone.
• 1,2 Because the rate of DNA migration is shifted or retarded upon protein binding, the assay is also referred to as a gel shift or gel retardation assay.
• Gel shift assay Also known as gel retardation assay or gel mobility shift assay.
• 12 Because the rate of DNA migration is shifted or retarded upon protein binding, the assay is also referred to as a gel shift or gel retardation assay.
• 1 ml, store at 4C (40 assays) GS Loading Solution: 1 ml, store at 4C Product Description: Gel shift assay, also known as gel retardation assay or electrophoretic mobility shift assay (EMSA), is widely used for the detection of DNA/RNA-protein complexes.