Gene targeting
From Health Encyclopedia
Contents |
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See for
homologous recombination, knockout gene
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Broader Terms
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Related Terms
gene expression, genetically modified animal, recombinant DNA
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Scope Note
genetic technique creating a recombinational event that produces exchange between DNA introduced into a cell and its homologous chromosomal target; involves two steps: pairing of DNA strands at the region of homology, and DNA strand exchange.
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Facts (generated by robot; please edit if you find it inaccurate)
- Gene targeting is enhanced in human cells overexpressing hRAD51.
- Gene targeting is locus dependent in the filamentous fungus Aspergillus nidulans.
- Gene targeting is the process by which an exogenous segment of DNA replaces an endogenous genomic target by homologous recombination.
- The advantage of gene targeting is that if the DNA sequence of the target gene is known, researchers can precisely knock out the gene at a high rate of efficiency.
- "Gene targeting is a method for making specific mutations in a gene of choice," says Dana Carroll, chair and professor of biochemistry at the University of Utah School of Medicine.
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