Immobilized enzyme
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Scope Note
enzymes which are immobilized on or in a variety of water-soluble or water-insoluble matrices with little or no loss of their catalytic activity; since they can be reused continuously, immobilized enzymes have found wide application in the industrial, medical and research fields.
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Facts (generated by robot; please edit if you find it inaccurate)
- And that is exactly what an immobilized enzyme is: an enzyme that is physically attached to a solid support over which a substrate is passed and converted to product.
- The activity of the immobilized enzyme is studied by measuring the amount of 6-APA released during the hydrolysis of 5% penicillin G at 37C and pH = 8.
- A sensor containing an enzyme as set forth in claim 28 wherein said membrane containing a first immobilized enzyme is directly coated and hardened on said pH-ion sensitive field effect transistor.
- The immobilized enzyme is specific for glucose and sufficiently long-lived for many sensor applications, but new glucose sensing applications may place increased demands on the enzyme.
- Therefore, characterization of the immobilized-enzyme is fundamental to the immobilization studies.
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