Immunoblotting
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- A new approach to the detection of anti-pituitary autoantibodies by immunoblotting is presented.
- Immunoblotting is good to use where only small quantities of sample are available as it can detect as little as picogram amounts of protein, depending on the specificity of the antibodies.
- Immunoblotting is useful when the antigen of interest is insoluble or readily degraded and cannot be easily immunoprecipitated.
- Immunoblotting is also used for the diagnosis of H.
- Immunoblotting is shown for the lysates (5% of input) or the precipitates.
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