Nucleic acid purification
From Health Encyclopedia
Contents |
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Used for
DNA purification, RNA purification
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Broader Terms
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Related Terms
genetic technique, nucleic acid quantitation /detection
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Scope Note
techniques and equipment used to purify nucleic acids.
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Facts (generated by robot; please edit if you find it inaccurate)
- Since nucleic acid purification is just the start of the sequencing protocol, any errors introduced at this stage can propagate throughout the rest of the procedure, wasting chemicals, time and effort.
- 4 QUANTITATIVE PCR P R O B L E M > S O L U T I O N > Nucleic acid purification is tedious, time consuming, and leads to the loss of precious RNA molecules, particularly low abundance messages.
- Nucleic acid purification is fully automated using the MagNA Pure LC Instrument in combination with the MagNA Pure LC DNA Isolation Kit I.
- Sequence-specific nucleic acid purification is typically achieved by hybridizing all or part of the DNA target to an immobilized probe, for example, an oligonucleotide grafted to a solid surface (9), a magnetic bead (10), or a polymer gel (11).
- Where nucleic acid purification is required, the lysis solution-treated cells are contacted with a nucleic acid capture matrix having an average pore size of at least about 1m.
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