Subtraction hybridization
From Health Encyclopedia
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Broader Terms
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Related Terms
representational difference analysis
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Scope Note
effectively subtracts the expressed mRNA complement from some baseline state or tissue from the expressed mRNA of the state or tissue of interest, leaving the fraction of mRNA which is uniquely expressed.
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Facts (generated by robot; please edit if you find it inaccurate)
- Subtraction hybridization is a technique that tells us what gene is present in L(gorillar) but absent in L(chimp), and vice versa.
- The reason that the LDL receptor was not found in by subtraction hybridization is unclear.
- Gene expression analysis using subtraction hybridization is a traditional technique to collect a number of differentially expressed genes (Jiang et al.
- Genetics RaSH, a rapid subtraction hybridization approach for identifying and cloning differentially expressed genes Hongping Jiang*, Dong-chul Kang*, Deborah Alexandre, and Paul B.
- Customized rapid subtraction hybridization (RaSH) gene microarrays identify overlapping expression changes in human fetal astrocytes resulting from human immunodeficiency virus-1 infection or tumor necrosis factor-alpha treatment.
- Chris Evelo, BiGCaT Bioinformatics BMT/TUE & UM Analyses of suppression, subtraction hybridization experiments Desciption: gene expression on the mRNA level be detected using so called suppression, subtraction hybridization.
- The down-regulation of gene expression was found to be a major effect of RV infection by using subtraction hybridization.
- My idea was to do a subtraction hybridization between T-cell cDNAs and B-cell RNA, but my grant was turned down because the NCI thought it wasnt feasible to do subtraction hybridization between two such similar cell types.
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