Transposase

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Use

transposon /insertion element

Facts (generated by robot; please edit if you find it inaccurate)

• 93, Issue 3, 1146-1150, February 6, 1996 Biochemistry The wing of the enhancer-binding domain of Mu phage transposase is flexible and is essential for efficient transposition (helix-turn-helix / mutagenesis / NMR / heteronuclear relaxation) Robert T.
• Transposase is the only nematode protein required for in vitro transposition of Tc1.
• 7590392 A purified mariner transposase is sufficient to mediate transposition in vitro DavidJ.
• If the transposase is no longer provided in trans then the transposon will be stable, so you do not have to worry about subsequent, secondary transposition events.
• Transposase is very unstable, so it is not normally accumulated to high concentrations.

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