Transposon element
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- Thus, when transposition occurs, the entire transposon element is excised from the chromosome, along with the antibiotic resistance gene.
- The dSpm transposon element is produced by the Arabidopsis Enhancer/Spm system (Tissier et al.
- The transposon element is of the colonist1 type and has previously been detected in the maize gene for acetyl-CoA carboxylase (Lutz and Gengenbach, 1996; accession no.
- The transposon element is a linear nucleic acid fragment that can be used as a linear fragment or circularlized, for example in a plasmid.
- To locate the insertion site of a transposon element, plasmid DNA was sequenced by the dideoxynucleoti 1e72 de termination method with Sequenase (United States Biochemical), using double-stranded DNA templates and the TnlacZ-II oligonucleotide primer (19).
- Besides, the most plasmids/BAC/PAC contain ampicillin , kanamycin or tetracyclin segment which provide recombination site for transposon element.
- Genetic analysis with the transposon element that caused insertional inactivation located the tdc gene at min 67 on the E.
- It is composed of the SB transposon element and the separately expressed transposase.
- Here, we are investigating the origin of the transposon element.
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