Transposon site hybridization
From Health Encyclopedia
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See
nucleic acid hybridization, transposon /insertion element
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- Koh and colleagues have employed transcription profiling, TrASH (transposon site hybridization), and bacterial mutagenesis techniques to identify virulence factors that are essential for colonization (e.
- The parental library of PA14 transposon insertion mutants was generated by using MAR2xT7, a transposon compatible with transposon-site hybridization and based on mariner.
- LI, Departments of Applied Oral Science and Microbiology and Immunology, Dalhousie University Title Construction of A Novel Transposon Delivery Vector Useful for Microarray-Transposon Site Hybridization in Gram-Positive Bacteria.
- This array will be used to perform expression analyses in different stress conditions but also to screen mutants generated by transposition using the TraSH methodology (Transposon Site Hybridization).
- Here, we describe a technique, transposon site hybridization (TraSH), which allows rapid functional characterization by identifying the complete set of genes required for growth under different conditions.
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