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i want to ask that is there any reducing of proteins during masturbuating
Yes dear.. u r assuming 100% right.
The white colored fluid/semen ejaculated through penis contains a rich amount of proteins/amino acids in it.
Human semen has a pH value of 7.05 - 8.0 & contains large amounts of glucose, calcium, acid soluble phosphorous, small amount of chloride.
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Since all males produce sperm 24/7, it makes no difference if it gets ejaculated or not. The amount you lose is negligible compared to your daily requirements, and there is no need to save it for later. Once you ejaculate. Your testicles start to produce more sperm. And the production of sperm takes quite a lot from your body therefore it's important to eat healthy and stay in shape. 
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